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To organize your image library, use Adobe Bridge (found on the Photoshop menu).
The following sections walk you through the basic functions of the tool. To learn more about specific functions, see Part III.
Creating a new file
Photoshop is a program that is often used to open source files (those created with other programs) and make minor changes or modifications.
You need to create a new document to do this. Most likely, you use Photoshop's New tool, found on the File menu at the top of the workspace window.
When you use the New tool, you have a number of options. To quickly create a new document, type a number in the New Document dialog box at the top of the workspace window. In the following example, you can use the document name of figure 8-9 (boston_0101), and set the Width to 610 pixels and the Height to 590 pixels.
This dialog box allows you to choose between several predesigned types of documents, such as RGB Color, Grayscale, Indexed, and CMYK.
Create a new document using the New Document dialog box.
To change a document, use the New Open dialog box, as shown in figure 8-10. (Choose File⇒New Open to open the box.) You have the option to use an existing file as the basis for the new document or to create a new document using the New Document dialog box.
**Figure 8-9:** Create a new document using the New Document dialog box.
**Figure 8-10:** Change the settings for the new document.
Before you can select a new document, you must add a new layer that represents the background in your design. You can add a new layer by pressing Ctrl+Shift+N on your keyboard or by choosing Layer⇒New Layer.
If you click OK in the dialog box, you see the screen shown in figure 8-11. You can see that the background is gray; it has a Fill layer so that you can change it to an image or color.
**Figure 8-11:** Create a new document with a background.
Next, you need to add at least one layer of color, bitmap, or text to the document. Choose Layer⇒New Layer. You can also add a new layer by pressing Ctrl+Shift+N on your keyboard.
Add a new layer
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The following Photoshop features are supported by Photoshop Elements:
7.0 and 8.0, 8.0 and 9.0 or higher
Quick selection tools
Window layouts and interface
Pixel maps and masking
Merging and splitting layers
3D rotation and perspective control
3D painting tools
Shadows and highlights
Save for Web and Devices
Photo & Video Transitions
Photo editing tools
Adjustment layers and tools
Mask and layer effects
Vector editing options
Import & export
Notes, bullet lists, tables
Vector pattern tools
Image retouching and effects
Anaglyph and polarizing filter
Text, illustrations and effects
Fitting text in layers
Convert to outlines
Stabilize digital photos
What’s new in Photoshop Elements:
New features that you can use in Photoshop Elements:
Retouch your photos, easily. The whole process is easy thanks to Photoshop Elements’ retouching features. Photoshop Elements offers a host of tools to help you get the best results.
In the Adjustments panel, click the Layers button to make changes directly to the original image’s layers instead of to the original pixels.
You can add effects to layers such as blur, vignette, contrast, luminosity, saturation, color, grayscale, tone mapping, embossing, and others.
You can isolate the changes you make to a specific layer, even while working on other images in the same folder.
Here are a few more features:
Create Flash animations and more with Adobe Flash Professional.
New and improved features in Adobe Illustrator
Get advanced vector graphics with Adobe Illustrator
Get better creative tools with advanced art and design technology with the Creative Cloud.
Keep up with the newest features and innovations for Adobe InDesign
For more information about Adobe’s software updates and what you can expect from them, read the Adobe blog.
Time for a new computer? See how quickly your digital life can be up and running with a Macbook.
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Specific protein-DNA interaction has been implicated in many important biological processes, including the replication of DNA, cell cycle progression, and genetic recombination. Recently, it has been shown that, in addition to DNA-binding proteins and DNA-binding sequences, the cellular environment contains a large number of proteins that specifically bind DNA sequences. These proteins have been proposed to play an important role in the regulation of gene expression. For example, it has been shown that heat shock proteins, which are present in all cells, can bind specifically to DNA and regulate the transcription of certain heat shock genes. The expression of these genes is thought to be controlled by a combination of protein-DNA interactions and the presence of transcription factors.
Because the interactions between regulatory proteins and their DNA target sites generally are non-specific and require in vitro conditions that cannot be easily duplicated in vivo, it is difficult to identify and map specific protein-DNA interaction sites.
Current methods of identifying specific protein-DNA interaction sites include the ligation in situ method described by Pilch et al., Proc. Natl. Acad. Sci. USA, 85: 9279 (1988), and the selective protein-DNA affinity chromatography described by Burton et al., Science, 243: 1544 (1989) and Krenz et al., Genes & Devel., 3: 431 (1989).
In the ligation in situ method, a partial DNA sequence is hybridized to a DNA sequence on a DNA fragment that includes a restriction enzyme recognition site at one end and a ligation site at the other end. When an RNA polymerase transcribes the sequence, it forms RNA-DNA hybrid molecules at the restriction site. This RNA molecule is specifically modified through the addition of a phosphonate group at the 3' end and bound to a solid substrate. DNA ligase can ligate the fragment only if the restriction site is in the same transcriptional orientation as the hybridization. This restricts the ligation to sites that are transcribed by the same RNA polymerase. Since the ligation is limited to sites that can be transcribed, one must depend on coincidental transcription and the polymeric nature of RNA. By analogy with the in vitro situation, the in situ method is expected to be inefficient.
The selective protein-DNA affinity chromatography method relies on the ability of proteins to form reversible complexes with duplex DNA. However, reversible complexes do not form with all protein-DNA interactions, particularly if interactions with the DNA are weak. Therefore, proteins having low affin
Windows Vista (Windows 7 and 8 will run it also but it may have some problems)
Windows Server 2003, 2008, 2012, 2016 and VMWare Server 2.0 and higher
Intel Pentium 4 or equivalent (Pentium 3 and newer are not supported)
1 GB RAM (minimum)
1 GB available hard-disk space
The graphics card should be at least a 128 MB or better, DirectX 8 or higher with latest drivers
DirectX 7 is not required. DirectX 9 and higher may work with some